DNA-In? Cell-Specific Transfection Reagents are a line of transfection products developed by the lead members of the scientific team that invented and developed the Lipofectamine? family of reagents. These cell-specific reagents offer maximum DNA delivery in targeted common cell lines.
The cell-specific DNA-In? 293 Transfection Reagent provides maximum high transfection, expression levels and cell viability in HEK293 cells.
Product sheet
Features
Fast, simple, easy-to-follow protocol
One-step process: simply add DNA-In? 293 Transfection Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells
Minimal toxicity - no need to replace medium after transfection
During its development and validation process, DNA-In? 293 Transfection Reagent underwent extensive testing in many primary and cultured cells. In a head-to-head comparison study, DNA-In? 293 Transfection Reagent consistently outperformed Lipofectamine? 2000 and Lipofectamine? 3000 transfection reagents.
The following charts show side-by-side comparisons of DNA-In? 293 Transfection Reagent , Lipofectamine? 2000 (LF2K) and Lipofectamine? 3000 (LFK) in HEK293 cells:
DNA-In? 293 Transfection Reagent provides Maximum Transfection Efficiency in HEK293 cells
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Above, HEK293 cells were plated at 70% confluency and transfected with the EF-1 alpha promoter-GFP 24 hours after plating. DNA-In? 293 transfected cells (top image) provides much improved transfection efficiency and cell viability as compared to LF3K transfected cells (bottom image).
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