NEW DNA-In? CRISPR Transfection Reagent  

Specifically formulated for CRISPR/Cas9 transfection reagent

DNA-In? CRISPR transfection reagent is optimized for large plasmid delivery of CRISPR/Cas9 vectors  into a range of cell types. This reagent is especially well-suited for hard-to-transfect primary cells.  Below, a Cas9-GFP plasmid was delivered into primary fibroblasts, muscles cells, keratinocytes and others primary cells and commonly used cell types with high efficiency and low toxicity.

Highly efficient Cas9 delivery in primary cells

 Human dermal primary fibroblasts (above) were plated  to give ~70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In? CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 

 Human primary keratinocytes (above) were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In? CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K , L3K). 

 C2C12 mouse myoblasts  (above) were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In? CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 

 Human skeletal muscle cells (above) were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In? CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 

Features

• Optimized for large plasmid DNA containing Cas9/GFP/ guideRNA ( all-in-one).

• High efficiency delivery of Cas9 expression vectors in hard-to-transfect primary cells.

Technical Data

• Product Protocol

DNA-In? is registered trademarks of Molecular Transfer, Inc.

Lipofectamine?, Opti-MEM? are registered trademarks of Thermo Fisher Scientific Inc.