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Biontex品牌METAFECTENE? PRO是針對哺乳動物細胞的新一代轉染試劑
作者:moreybio 發布時間:2020-12-03 點擊次數:3923
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METAFECTENE? PRO

METAFECTENE? PRO是針對哺乳動物細胞的新一代轉染試劑。采用毒性優化專利技術(TOP技術),Biontex成功地降低了轉染試劑對細胞的毒性,并顯著提高DNA進入細胞的效率。除了能加強細胞的轉染效率之外,METAFECTENE? PRO的另一個主要特點是提高細胞內基因的表達。

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TOP技術(Toxicity Optimization Module)

注:TOP技術(Toxicity Optimization Module)

毒性最小化是現代轉染試劑達到最高效率和廣泛應用性的首要前提條件。TOP技術的基本準則是模擬物質天然結構,加強生物降解能力,提高細胞耐受性。另外,通過物理化學修飾,降低脂質復合體的穩定性,從而促進DNA在細胞質的釋放。

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Biontex產品

產品名稱

貨號

規格

METAFECTENE? PRO轉染試劑

T040-1.0


1 ml 

METAFECTENE? PRO轉染試劑

T040-2.0

2 X 1 ml

METAFECTENE? PRO轉染試劑

T040-5.0

5 X 1 ml

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HIGHLIGHTS:

Suitable for DNA, mRNA, siRNA and miRNA transfection as well as cotransfection of DNA and RNA

Suitable for stable and transient transfection

Suitable for adherend and suspension cells

Minimized toxic effects

Optimized transfection rates

Free from serum inhibition

Comprehensive library of user reports

Wide range of references in specialist literature

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METAFECTENE? PRO is a liposome based transfection reagent consisting of a mixture of polycationic and neutral lipids. It is an advanced product based on METAFECTENE?. Structural changes of the comprising cationic lipids results in higher efficiency and lower toxicity.

METAFECTENE? PRO is part of a temporal development series of transfection reagents from Biontex, which includes in particular the following reagents: METAFECTENE? < METAFECTENE? PRO < K2? Transfection System < K4? Transfection System. With the development degree the probability of a successful transfection increases (see also General Selection Aid).

Low cytotoxicity and lack of serum inhibition allow for a wide range of applications. METAFECTENE? PRO is suitable for transient and stable transfection of mammalian cell lines and primary cells with DNA (plasmids, bacmids), RNA (mRNA, miRNA and siRNA) and modified nucleic acids (antisense oligonucleotides). Areas of application are particularly the production of proteins, antibodies, and viruses (e.g. adenoviruses, AAV, lentiviruses), cotransfections of different nucleic acids, genome editing (e.g. CRISPR/Cas9, CRISPR/Cpf1) and gene silencing (gene knockdown).

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