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Bioo Scientific的NEXTflex? Rapid DNA-Seq Kit使得研究人員能夠在2小時(shí)內(nèi)從低至1ng的DNA完成適用于Illumina測(cè)序平臺(tái)的文庫構(gòu)建。該試劑盒是基因組DNA、FFPE樣品、ChIP DNA以及微量臨床樣本建庫的最佳選擇。
NEXTflex? Rapid DNA-Seq Kit在樣品的起始量上更加靈活,從1ng 到1ug的3個(gè)數(shù)量級(jí)范圍均是適用的。基于磁珠法進(jìn)行片段大小選擇的建庫流程免除了瓊脂糖電泳跑膠的步驟,同時(shí)多達(dá)384 種接頭barcode更加滿足了高通量測(cè)序的需求。
NEXTflex? Rapid DNA-Seq Kit采用增強(qiáng)的接頭連接技術(shù),提高了接頭的連接效率,得到更長(zhǎng)、更多樣的測(cè)序reads。Bioo Scientific的NEXTflex?試劑盒連接反應(yīng)和聚合酶反應(yīng)混合體系確保了高質(zhì)量文庫的構(gòu)建。
NEXTflex? Rapid DNA-Seq Kit Bundle
為了方便用戶訂購,Bioo Scientific同時(shí)提供包含了NEXTflex?快速DNA建庫試劑及NEXTflex?接頭barcode的試劑套裝。該套裝現(xiàn)有兩個(gè)不同的包裝。這兩個(gè)不同包裝都包含了48個(gè)樣本的NEXTflex?快速DNA建庫試劑和24個(gè)獨(dú)特的NEXTflex?接頭barcode(每個(gè)barcode對(duì)應(yīng)兩次反應(yīng))。
靈活的多重測(cè)序選擇
NEXTflex?的長(zhǎng)的、退火的、包含index序列的接頭設(shè)計(jì)不但進(jìn)一步改進(jìn)了混合文庫的建庫流程,也使得樣品的設(shè)置更加靈活。NEXTflex? Rapid DNA-Seq Kit的barcode對(duì)于單端、雙端以及混合文庫構(gòu)建都是適用的。NEXTflex Rapid DNA-Seq Kit 可選擇NEXTflex? DNA Barcodes,NEXTflex-96? DNA Barcodes,NEXTflex-HT? DNA Barcodes和NEXTflex? Dual-Indexed Barcodes用于大于等于10 ng起始量的樣品進(jìn)行文庫制備,也可選擇NEXTflex? ChIP-Seq Barcodes 和NEXTflex-96? ChIP-Seq Barcodes用于小于10ng起始量的樣品進(jìn)行文庫制備。
自動(dòng)化的DNA文庫構(gòu)建流程
Sciclone NGS/NGSx工作站的運(yùn)用可以讓使用 NEXTflex? Rapid DNA-Seq Kit的客戶獲得一個(gè)完美的DNA自動(dòng)化建庫流程。
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 規(guī)格 |
NOVA-5144-01 | NEXTflex? Rapid DNA-Seq Kit | 8 rxns |
NOVA-5144-02 | NEXTflex? Rapid DNA-Seq Kit | 48 rxns |
NOVA-5144-03 | NEXTflex? Rapid DNA-Seq Bundle - Barcodes 1-24 | 48 rxns |
NOVA-5144-04 | NEXTflex? Rapid DNA-Seq Bundle - Barcodes 25-48 | 48 rxns |
NOVA-514101 | NEXTflex? DNA Barcodes - 6 | 48 rxns |
NOVA-514102 | NEXTflex? DNA Barcodes - 12 | 96 rxns |
NOVA-514103 | NEXTflex? DNA Barcodes - 24 | 192 rxns |
NOVA-514104 | NEXTflex? DNA Barcodes - 48 | 384 rxns |
NOVA-514105 | NEXTflex-96? DNA Barcodes (in 96-well plate) | 768 rxns |
NOVA-514106 | NEXTflex-96? DNA Barcodes (in microfuge tubes) | 768 rxns |
NOVA-514160 | NEXTflex? Dual-Indexed DNA Barcodes 1-96 | 768 rxns |
NOVA-514161 | NEXTflex? Dual-Indexed DNA Barcodes 97-192 | 768 rxns |
NOVA-514170 | NEXTflex-HT? Barcodes - 6 (in tubes) | 48 rxns |
NOVA-514174 | NEXTflex-HT? Barcodes 1-96 (in plates) | 192 rxns |
NOVA-514175 | NEXTflex-HT? Barcodes 97-192 (in plates) | 192 rxns |
NOVA-514176 | NEXTflex-HT? Barcodes 193-288 (in plates) | 192 rxns |
NOVA-514177 | NEXTflex-HT? Barcodes 289-384 (in plates) | 192 rxns |
NOVA-514150 | NEXTflex? Unique Dual Index Barcodes – 1 - 96 (in 96-well plate) | 192 rxns |
NOVA-514151 | NEXTflex ? Unique Dual Index Barcodes – 97 - 192 (in 96-well plate) | 192 rxns |
NOVA-514120 | NEXTflex? ChIP-seq Barcodes - 6 | 48 rxns |
NOVA-514121 | NEXTflex? ChIP-seq Barcodes - 12 | 96 rxns |
NOVA-514122 | NEXTflex? ChIP-seq Barcodes - 24 | 192 rxns |
NOVA-514123 | NEXTflex? ChIP-seq Barcodes - 48 | 384 rxns |
NOVA-514124 | NEXTflex-96? ChIP-seq Barcodes - 96 | 768 rxns |
文獻(xiàn)引用
Shain, A. H., et al. (2015) The Genetic Evolution of Melanoma from Precursor Lesions. The New England Journal of Medicine. 373:1926-1936. doi: 10.1056/NEJMoa1502583.
Gal, C. et. al. (2015) The impact of the HIRA histone chaperone upon global nucleosome architecture. Cell Cycle. 14:1, 123-134. doi:10.4161/15384101.2014.967123.
Laver, T., Harrison, J., O’Neill, P. A., Moore, K., Farbos, A., Paszkiewicz, K. and Studholme, D. J. (2015) Assessing the performance of the Oxford Nanopore Technologies MinION. Biomolecular Detection and Quantification 3 (2015) 1–8. doi :10.1016/j.bdq.2015.02.001.
Yang, Y. A., et al. (2016) FOXA1 potentiates lineage-specific enhancer activation through modulating TET1 expression and function. Nucleic Acids Research. doi: 10.1093/nar/gkw498
Palomo, A., et al. (2016) Metagenomic analysis of rapid gravity sand filter microbial communities suggests novel physiology of Nitrospira spp. The ISME Journal. doi:10.1038/ismej.2016.63.
Brodie, J., et al. (2016) Characterising the microbiome of Corallina officinalis, a dominant calcified intertidal red alga. FEMS Microbiology Ecology. doi: 10.1093/femsec/fiw110.
Givnish, T. J., et al. (2016) Phylogenomics and historical biogeography of the monocot order Liliales: out of Australia and through Antarctica. Cladistics. doi: 10.1111/cla.12153.
Harrisson, K., et al. (2016) Pleistocene divergence across a mountain range and the in?uence of selection on mitogenome evolution in threatened Australian freshwater cod species. Heredity. 1–10. doi:10.1038/hdy.2016.8.
默瑞(上海)生物科技有限公司(Morey Biosciences ,Inc .簡(jiǎn)稱默瑞生物)成立于2016年7月22日,其 團(tuán)隊(duì)具有豐富的生命科學(xué)行業(yè)經(jīng)驗(yàn)和強(qiáng)大的技術(shù)服務(wù)能力。
“創(chuàng)新,專業(yè),共贏”,默瑞生物以此為經(jīng)營(yíng)理念,不斷強(qiáng)化管理體系,提升客戶體驗(yàn),實(shí)現(xiàn)多方共贏。
默瑞生物擁有兩個(gè)銷售團(tuán)隊(duì),基礎(chǔ)科研組和企業(yè)應(yīng)用組。針對(duì)不同的客戶需求點(diǎn),提供更為專業(yè)全面的產(chǎn)品和服務(wù)。
我們的自產(chǎn)和代理產(chǎn)品,全面滿足分子、細(xì)胞、組織、胚胎和個(gè)體水平的研究,廣泛應(yīng)用于基礎(chǔ)科研、技術(shù)轉(zhuǎn)化、應(yīng)用檢測(cè)、研發(fā)生產(chǎn)等廣泛領(lǐng)域。我們將竭盡所能為生命科學(xué)和醫(yī)學(xué)事業(yè)的發(fā)展提供更好的產(chǎn)品,更先進(jìn)的技術(shù)和更專業(yè)的服務(wù)。
默瑞(上海)生物科技有限公司
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