mNeonGreen-Trap用于免疫共沉淀反應(yīng)
pulldown mNeonGreen融合蛋白的優(yōu)越性能
·對(duì)于下游應(yīng)用無(wú)重輕鏈污染
·強(qiáng)力洗滌條件下，依舊能牢牢結(jié)合
·孵化時(shí)間短至30min
mNeonGreen-Trap
ChromoTek mNeonGreen-Trap 經(jīng)過(guò)驗(yàn)證，對(duì)于細(xì)胞或組織中提取的mNeonGreen融合蛋白及其相互作用物的快速、有效一步法免疫沉淀，是一種卓越的工具。mNeonGreen-Trap包含了一個(gè)羊駝單域抗體偶聯(lián)至瓊脂糖珠或磁性瓊脂糖珠。
I input , FT: Flow-through, B: Bound. Coomassie gel and Western blot. Western blot indicates high effectiveness of pull-down
應(yīng)用
·免疫沉淀(IP)/免疫共沉淀(Co-IP)
·質(zhì)譜分析
·酶活性檢測(cè)
·CHIP/RIP分析
強(qiáng)大而穩(wěn)定的結(jié)合能力
·高親和力，最低能與解離常數(shù)為2nM結(jié)合
·能承受的清洗條件：6M urea, 2M NaCl, 10mM DTT, 2% Nonidet P40 Substitute, 1% Triton X-100, 0.2% SDS
特性
·mNeonGreen-Trap識(shí)別的抗原決定基團(tuán)與anti-mNeonGreen 鼠多抗不同
·高結(jié)合能力，每10μl懸濁液中加入12μg mNeonGreen
·便捷——有瓊脂糖珠與磁性瓊脂糖珠可供選擇
技術(shù)
駱駝科，比如羊駝，具有一種重鏈抗體。這些重鏈抗體缺乏輕鏈抗體，并通過(guò)單結(jié)構(gòu)域(VHH)結(jié)合至抗原，這個(gè)單結(jié)構(gòu)域也被稱為nanobody。這些VHH結(jié)構(gòu)域具有出色的結(jié)合特性并能保證批次間的穩(wěn)定性。
anti-mNeonGreen IgG 抗體
mNeonGreen的免疫熒光(IF), 免疫印跡(Western Blot)以及ELISA實(shí)驗(yàn)
敏感性：檢測(cè)低表達(dá)的蛋白
經(jīng)確認(rèn)的特異性鼠多抗
anti-mNeonGreen IgG antibody 32F6
ChromoTek品牌的anti-mNeonGreen antibody 32F6是經(jīng)過(guò)驗(yàn)證的，能在Western Blot及免疫熒光實(shí)驗(yàn)中敏感檢測(cè)出mNeonGreen融合蛋白的卓越工具。anti-mNeonGreen antibody是一種純化的mouse monoclonal IgG2a。
特性
與mNeonGreen-Trap的antimNeonGreen VHH識(shí)別的抗原不同。
高特異性與親和力，最低能與解離常數(shù)為3.5nM結(jié)合
免疫熒光(IF)
Immunostaining of Hela cells transiently expressing mNeonGreen fused to Actin Chromobody (green) with 32F6 antibodies (red). Merge image shows overlay of green and red channels and DAPI (blue). Scale bar, 10μm.
Western Blot (WB)
Western blot analysis of cell extract from HEK293T cells transiently expressing mNeonGreen-beta-Actin (68.7 kDa).
ELISA
HRP signal is detected as normalized absorption in presence (+, strong signal) and absence (-, faint signal) of anti-mNeonGreen VHH. Result shows the applicability of this antibody pair for ELISA measurements.
